flow cytometry

flow cytometry
Slightly imprecise but common term for the use of the Fluorescence Activated Cell Sorter (FACS). Cells are labelled with fluorescent dye and then passed, in suspending medium, through a narrow dropping nozzle so that each cell is in a small droplet. A laser-based detector system is used to excite fluorescence, and droplets with positively fluorescent cells are given an electric charge. Charged and uncharged droplets are separated as they fall between charged plates, and so collect in different tubes. The machine can be used either as an analytical tool, counting the number of labelled cells in a population, or to separate the cells for subsequent growth of the selected population. Further sophistication can be built into the system by using a second laser system at right angles to the first to look at a second fluorescent label, or to gauge cell size on the basis of light-scatter. The great strength of the system is that it looks at large numbers of individual cells, and makes possible the separation of populations with, for example, particular surface properties.

Dictionary of molecular biology. 2004.

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  • flow cytometry — flow cytometry. См. проточная цитометрия. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

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  • Flow cytometry — Analysis of biological material by detection of the light absorbing or fluorescing properties of cells or subcellular fractions such as chromosomes passing in a narrow stream through a laser beam. Flow cytometry can be used with automated sorting …   Medical dictionary

  • flow cytometry — noun : a technique for identifying and sorting cells and their components (as DNA) by staining with a fluorescent dye, detecting the fluorescence usually by passing the cells individually in solution through laser beam illumination, and counting… …   Useful english dictionary

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  • flow cytometry — noun Date: 1978 a technique for identifying and sorting cells and their components (as DNA) by staining with a fluorescent dye and detecting the fluorescence usually by laser beam illumination • flow cytometer noun …   New Collegiate Dictionary

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  • flow cytometry — noun A technique used to sort and classify cells by using fluorescent markers on their surface …   Wiktionary

  • flow cytometry — a technique in which cells are tagged with a fluorescent dye and then directed single file through a laser beam. The intensity of fluorescence induced by the laser beam is proportional to the amount of DNA in the cells …   The new mediacal dictionary

  • flow cytometry — A method of measuring the number of cells in a sample, the percentage of live cells in a sample, and certain characteristics of cells, such as size, shape, and the presence of tumor markers on the cell surface. The cells are stained with a light… …   English dictionary of cancer terms

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